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http://ciateq.repositorioinstitucional.mx/jspui/handle/1020/639| Evaluation of Poly-3-Hydroxybutyrate (P3HB) scaffolds used for epidermal cells growth as potential biomatrix | |
| Sandra Garcia Cerna Angelica Meneses Acosta JOSE MANUEL ROJAS GARCIA Bernardo Campillo DANIEL GENARO SEGURA GONZALEZ Carlos Peña | |
| Acceso Abierto | |
| Atribución-NoComercial-CompartirIgual | |
| https://doi.org/10.3390/polym14194021 2073-4360 https://www.mdpi.com/journal/polymers https://www.mdpi.com/2073-4360/14/19/4021 | |
| P3HB scaffolds Tissue engineering Keratinocyte 3D printing | |
| Otro autor: Uriel Sánchez Pacheco. Advances in tissue engineering have made possible the construction of organs and tissues with the use of biomaterials and cells. Three important elements are considered: a specific cell culture, an adequate environment, and a scaffold. The present study aimed to develop P3HB scaffolds by 3D printing and evaluate their biocompatibility with HaCaT epidermal cells, as a potential model that allows the formation of functional tissue. By using a method of extraction and purification with ethanol and acetone, a biopolymer having suitable properties for use as a tissue support was obtained. This polymer exhibited a higher molecular weight (1500 kDa) and lower contact angle (less than 90°) compared to the material obtained using the conventional method. The biocompatibility analysis reveals that the scaffold obtained using the ethanol–acetone method and produced by 3D printing without pores was not cytotoxic, did not self-degrade, and allowed high homogenous cell proliferation of HaCaT cells. In summary, it is possible to conclude that the P3HB scaffold obtained by 3D printing and a simplified extraction method is a suitable support for the homogeneous development of HaCaT keratinocyte cell lineage, which would allow the evaluation of this material to be used as a biomatrix for tissue engineering. | |
| MDPI | |
| 2022 | |
| Artículo | |
| Polymers, vol. 14, no. 19, p. 4021. | |
| Inglés | |
| Público en general | |
| García-Cerna, S., Sánchez-Pacheco, U., Meneses-Acosta, A., Rojas-García, J., Campillo-Illanes, B., Segura-González, D., & Peña-Malacara, C. (2022). Evaluation of poly-3-hydroxybutyrate (P3hb) scaffolds used for epidermal cells growth as potential biomatrix. Polymers, 14(19), 4021. https://doi.org/10.3390/polym14194021 | |
| OTRAS | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos Publicados en Revistas Indexadas |
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| Evaluation of poly-3-hydroxybutyrate.pdf | 3.64 MB | Adobe PDF | Visualizar/Abrir |